Baltimore City Fire released the names of the mother and two children killed in Wednesday morning's fire on East Coldspring Lane.
Alicia Evans, 26, Amani Mullen, 5, and Layla Mullen, 4, died from thermal injuries and smoke inhalation.
Evans and Layla Mulled died in the fire. Amani Millen was taken to the hospital where she passed away on Thursday.
"Our heartfelt thoughts and prayers continue to go out to this family during this difficult time," said Chief Niles R. Ford.
The cause of the fire remains under investigation.
So far this year, there have been 28 fire fatalities in Baltimore City.